Establishment of orthotopic transplantation model of MKN-45 human gastric cancer and evaluation of the efficacy of paclitaxel in this model

Abstract

Objective To establish an orthotopic transplantation model of bioluminescent human gastric carcinoma and visually evaluate the therapeutic effect of paclitaxel in this model using in vivo imaging system. Methods The intact MKN-45-luc tumor tissue, stably expressing luciferase, was implanted into the gastric serosa of nude mice by orthotopic transplantation technique according to the subcutaneously implant-passage-orthotopic transplantation mode. Each mouse was photographed by in vivo fluorescence imaging system at the 7th and 14th day after surgery to evaluate the tumorigenecity. Paclitaxel was administered intravenously on tumor-bearing mice at the dose of 10 mg/kg. The changes of fluorescence intensity and weight were observed every 4 days. The fluorescence-positive mice were sacrificed after 26 days, and hematoxylin and eosin (HE) staining was performed to observe the morphology of endogastric xenograft tumor and metastasis. Results The morphology and paclitaxel sensitivity of MKN-45-luc were consistent with MKN-45 (P>0.05). The orthotopic model was successfully established, giving the successful rate of 90% (18/20), and lymph node metastasis and liver metastasis were observed. The histological characteristics of the tumor tissues from the model after HE staining were the same as gastric cancer. Compared with the control group, paclitaxel had a significant anti-tumor effect after administration on this mice model. Conclusion It is convenient and efficient to establish the orthotopic transplantation model of gastric cancer using gastric bursa-OB glue method. Key words: Gastric cancer; Orthotopic transplantation model; Gastric bursa-OB glue method; Paclitaxel; Living bioimaging