Establishment of orthotopic transplantation model of malignant glioma masses of human brain in nude mice

Abstract

Objective To establish an orthotopic transplantation model of malignant glioma of human brain in nude mice,and analysize its biological features.Methods Fresh anaplastic astrocytorna masses with expression of EGFR from human brain were collected and orthotopicly transplanted into the right caudate nucleus of nude mice.The newly formed xenografts were sharply mineed into pieces and re-transplanted.HE staining and MRI scanning were performed to investigate the histopathological and imaging features of the xenografts.The expression of EGFR in xenografts was also investigated by immunohistochemical assays ofEnVision.Results Histopathologically,the cerebral xenografts were pnody differentiated anaplastic astrocytoma with invasiveness to normal brain tissue.MRI revealed a poor-delimitated mass with surrounding tissues,which perfectly recapitulated imaging features of the original tumor.Expression of EGFR was positive in the cerebral xenografts with the ratio of 70%.Conclusions The orthotopic xenografts of brain anaplastic astrocytoma could faithfully recapitulate the biological features of the original tumor clinically and pathologically,and provides a reliable animal model not only for studying the biological features but also for experimental treatment of brain malignant glioma. Key words: Anaplastic astrocytoma; Nude mice; Orthotopic xenograft; Invasive growth; ECFR