Abstract
SPECIFIC AIMSWe tried to establish serum-free culture conditions for primary as well as immortal p53-deficient murine erythroblasts, which should allow to study sustained proliferation as well as maturation of immature cells into enucleated erythrocytes in precisely defined combinations of proliferation or differentiation factors. If it could be verified that such a cell system faithfully executes essential steps of normal erythropoiesis, this would permit characterization of molecular mechanisms involved in red cell maturation by expression profiling.PRINCIPAL FINDINGSIn vitro culture, phenotypical characterization, and differentiation of primary mouse erythroblastsDetailed molecular characterization of terminal erythropoiesis in the mouse has so far been hampered by the lack of suitable in vitro culture models. The available primary cell systems suffer from insufficient immature cell numbers and from the heterogeneity of these cells with respect to stage of maturity. In contrast, immortal erythroleukemi...
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