Comparison of calcium mobilization in response to noradrenaline and acetylcholine in submandibular cells of newborn and adult rats

Abstract

The response of mature and immature rat submandibular cells to α-receptor stimulation was compared in terms of the generation of inositol triphosphate (IP 3) and Ca 2+ mobilization, and of how the calcium mobilization response affects acetycholine (ACh)-induced Ca 2+ mobilization. In mature cells, noradrenaline (NA) caused much smaller IP 3 and Ca 2+ responses than ACh. However, the Ca 2+ release induced by NA was enough to partially discharge an agonist-sensitive store and to reduce Ca 2+ release by a subsequent ACh stimulus. Exposure to NA also caused an influx of Ca 2+ in the mature cells, which was largely associated with Ca 2+ entry induced by store depletion (i.e. capacitative entry). In the immature submandibular cells of newborn rats, NA caused essentially no IP 3 response and a small Ca 2+ release, which only partially affected the Ca 2+ released by a subsequent exposure to ALCh. In contrast to adult cells, immature cells did not show an increased Ca 2+ influx after exposure to NA. However, prestimulation with this agonist potentiated the Ca 2+ influx activated by ACh in the calls of newborn rats, but not in cells of adult rats. As both mature and immature submandibular cells have a well-developed phosphoinositide turnover response to ACh, the findings in mature cells suggest a less efficient coupling between α-receptors and phospholipase C, while those in immature cells suggest that this coupling is even less functional in the early stages of postnatal development. In permeabilized and 45Ca 2+-loaded mature cells, cyclic ADP-ribose (cADPR) released 13.4% of loaded 45Ca 2+ and this release was significantly reduced by pre-exposure to IP 3. Similarly, pre-exposure to cADPR also reduced the IP 3-induced 45Ca 2+ release. It is concluded that: (I) stimulation with NA induces a smaller Ca 2+ release in mature and immature submandibular cells than ACh; (2) the mediator for this small Ca 2+ mobilization may be cADPR; and (3) NA stimulates capacitative Ca 2+ entry in mature cells, but not in immature cells, and it also activates a Ca 2+ entry pathway distinct from the one induced by store depletion, particularly in immature cells.