Abstract
Isolated root cultures which could be maintained over several months by serial subculture were established from Brassica oleracea var. italica cv. Green Comet Ft. A modified White's medium was found to be the best of several salt compositions tested. The effects on isolated root growth of the following were also examined; nutritional components, culture vessel type and closure, pH of the medium and auxin type and concentration. Using a medium devised for Green Comet, root cultures were established from six other B. oleracea , B. napus and B. campestris cultivars. It was possible to regenerate shoots from segments of cultured roots by incubation on agar-solidified media containing cytokinin and auxin. The effects on regeneration of various auxins and cytokinins were investigated; the combination of Picloram with KN gave the highest frequency of shoot formation. It was demonstrated that isolated roots retained their regenerative ability over a period of 5 months in culture.
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