ESTABLISHMENT OF IN VITRO PROPAGATION OF Hibiscus cannabinus (KENAF)

Nor Syafawati Mohamad Pauzi,Nurul Ain Saipul Bahari,Zarina Zainuddin

doi:10.26480/gws.01.2021.05.07

Abstract

Hibiscus cannabinus or commonly known as kenaf is a versatile plant that serves as resources for numerous manufacturing and livestock industries. Originally planted in West Africa, kenaf is now distributed in many countries including Malaysia as its fibres were proved to be an ultimate alternative resource for major industries such as automotive, paper and bio-composite. In fact, in Malaysia, due to its adaptation to wide range of climatic conditions, kenaf has potentially be chosen as a new industrial crop replacing tobacco. There have been many interests on regenerating kenaf via micropropagation as the demand for this crop has been increasing tremendously since the past decades. Hence, this study is initiated with the objective to establish in vitro propagation system of H. cannabinus. The callus induction was achieved on Murashige and Skoog (MS) media supplemented with different concentrations of benzylaminopurine (BAP). It was observed that calli were successfully induced on all the BAP concentrations tested. The optimum concentration of BAP that induced the healthiest and biggest calli was 3.0 mg/l. Shoot and root induction from the calli were attempted using MS medium supplemented with different combinations and concentrations of IBA, BA and GA3. From the seven treatments, three treatments successfully induced formation of shoot; treatment T3 (MS + 1.0 mg/l IBA + 2.5 mg/l BA), treatment T5 (MS + 0.1 mg/l IBA + 2.0 mg/l BA + 0.3 mg/l GA3) and treatment T6 (MS + 1.0 mg/l IBA + 2.5 mg/l BA + 0.3 mg/l GA3). The results obtained in this study can paved for more research on tissue culture of H. cannabinus.

Highlights

Hibiscus cannabinus or locally known as kenaf has many hidden benefits and remarkable qualities to both economy and industries that very little had known
The formation of callus for H. cannabinus was induced by implementing a range of BAP concentrations in the Murashige and Skoog (MS) media
Based on the results obtained, there was no indication of callus formation on the leaf explants cultured in the MS media without BAP, instead browning on the surface of the explants was noticed after 2 weeks of culture

Summary

Introduction

Hibiscus cannabinus or locally known as kenaf has many hidden benefits and remarkable qualities to both economy and industries that very little had known. This annual and fast-grown plant belongs to the family Malvaceae (Ibrahim et al, 2014). Originated from Sub-Saharan Africa, kenaf is predominantly distributed in Asia and Latin America (Ayadi et al, 2017; Xia et al, 2017). This plant has been aggressively planted due to its various range of uses from basic animal feed to production of biocomposite products such as ropes, canvases and carpets. Due to its undeniable potential contribution towards an improved country’s economy and society’s life, an effort to produce an efficient reproducible plant regeneration procedure for H. cannabinus plant has to be explored

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