Abstract
Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora. To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.
Highlights
Helicteres isora L. (Malvaceae) is a traditional medicinal plant, commonly referred as Indian Screw Plant
Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices
MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora
Summary
Helicteres isora L. (Malvaceae) is a traditional medicinal plant, commonly referred as Indian Screw Plant. The pods of this shrub have demonstrated many valuable activities including anti-plasmodial activity [2], hypoglycemic activity, hepatoprotective activity [3], antihyperglycemic and hypo-lipidemic activities [4,5], antinociceptive activity [3], antioxidant potencies [6], antimicrobial resistance reversal activities [7] and anti-HIV activity [8] This plant is being reported to be applicable in type II diabetes treatment [9]. The intense commercial and medicinal significance of H. isora implies the necessity of the establishing of the cell suspension culture of this plant for biomass as well as secondary metabolite production. We report the efficient procedure to attain healthy cell suspensions from callus of H. isora which can be explored for in vitro production and extraction of secondary metabolites
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