Establishment of calcium oxalate calculus model in drosophila induced by ethylene glycol

Abstract

Objective There is not any report on the Drosophila model of calcium oxalate calculus in China yet. We performed this study to develop a Drosophila model of calcium oxalate calculus, and to evaluate the stability and applicability of the model. Methods 400 same days old male Drosophila melanogaster were randomly divided into four groups: group A (n1=100), group B (n2=100), group C (n3=100) and group D (n4=100). After carbon dioxide anesthesia, they were transferred into conical flask with medium with 0.50%, 0.75%, 1.0% ethylene glycol and normal culture medium respectively. Drosophila were raised in 25 ℃ incubator, and their life span was recorded. After dissection of the Malpighi tubule, the crystal formation of the Drosophila was observed. Then the crystals were obtained and analyzed. Results The crystallization rates of groups A, B, C and D were 87%, 92%, 98% and 6%, respectively (χ2=273.057, P=0.000). The median life span of group A, B, C, D was 36 days, 31 days, 23 days and 52 days respectively, one-way ANOVA showed that F=35.403 and P=0.000. The crystals consisted of calcium oxalate. Conclusion 0.75% of the ethylene glycol concentration is considered to be the ideal concentration due to its high rate of stone formation, the relatively low mortality. In addition, The model is suitable for large sample volume drug test which make big sense for urolithiasis study. Key words: Urolithiasis; Drosophila melanogaster; Malpighi tubule; Ethylene glycol; Model, animal