Experimental renal calcium oxalate calculus models without severe renal damage in rats

Abstract

Objective To screen a short-term,economical and satisfied model of renal calcium oxalate calculus in rats with minimal renal damage.Methods Ninety male Sprague-Dawley (SD) rats were randomly divided into two groups.In group 1,the rats were treated with 1.0％ ammonium chloride and different concentrations of ethylene glycol (0.1％,0.2％,0.4％ and 0.8％).In group 2,the rats were treated with different concentrations of ethylene glycol (0.1％,0.2％,0.4％ and 0.8％).The body mass change,the serum creatinine and urine creatinine,and urinary oxalate excretion were measured.Conventional HE dyeing was carried out for kidney tissues and pathological changes,and the status of kidney stone formation was further observed.Results In group 1 treated with 1.0％ ammonium chloride and 0.1％ ethylene glycol for 7 days,the urinary oxalate excretion days was significantly higher than in control group (P ＜0.05).In group 1 treated with 0.4％ or 0.8％ ethylene glycol + 1.0％ ammonium chloride for 14 days,the creatinine clearance was lower than in control group (P ＜ 0.01).Light microscopy showed that calcium oxalate crystals formed in renal cortex,medulla and renal papilla,and inflammatory damage of renal tissue was aggravated.In group 2 treated with 0.4％ or 0.8％ ethylene glycol for 7 days,the urine oxalate excretion was higher than in control group (P ＜0.01),however,there was no statistically significant difference in the creatinine clearance (P ＞ 0.05).Light microscopy showed no obvious interstitial edema in renal cortex and a small amount of stone crystals were seen with no inflammatory cells infiltration.Conclusion Ethylene glycol alone is a quich,economic and good calcium oxalate crystallized method in rats without severe renal damage. Key words: Urolithiasis ; Calcium oxalate ; Ethylene glycol; Model,animal