Abstract
A rapid two-site enzyme immunoassay (EIA) for human epidermal growth factor (hEGF) was developed. Our rapid EIA was based on the sandwiching of an antigen between anti-hEGF IgG-coated polystyrene beads and anti-hEGF Fab'-linked peroxidase (horseradish peroxidase, EC. 1.11.1.7). This method is so rapid that we can get the results within 5h.The reproducibility of within- and between-assay was 4.8 to 7.1% and 3.5 to 7.2%, respectively. And discriminatory sensitivity was as low as 5pg/ml. The recovery of hEGF from urine and serum by this method was approximately 100%, and the serial dilution curves of unextracted human urine and serum samples were parallel with that of standard hEGF (Urogastrone).We applied this method to assay of hEGF content in urine of patients with a variety of tumors.
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