Abstract
目的 使用旋转细胞培养系统(rotating cell culture system,RCCS)模拟微重力环境,建立胰腺癌肝转移的体外模型.方法将人肝组织块和人胰腺癌细胞株BxPC-3置于旋转细胞培养系统中混合培养,在额定时间点取肝组织块送病理,HE染色观察肝组织结构和胰腺癌细胞在肝组织块中生长情况.结果混合培养第1天人肝组织块周围和管腔内即有胰腺癌细胞粘附并聚集;第3天可见肝组织块中有散在癌细胞浸润;第7天可见微小转移灶形成;混合培养第10天胰腺癌肝转移灶、肝细胞形态和肝小叶结构可保持完好,并可见癌组织块形成.结论使用旋转细胞培养系统模拟微重力环境,构建胰腺癌肝转移体外模型,可以用于胰腺癌肝转移过程和机制的研究,为研究胰腺癌细胞和肝组织间的相互关系提供新的方法。
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