Establishment of red fluorescent protein nude mice metastasis models of pancreatic cancer

Abstract

Objective To estabhsh stable high RFP-expressing nude mice metastasis models of pancreatic cancer. Methods RFP gene was transfected into human pancreatic cancer ceils SWi990. The biological behavior of the two cell lines was assessed. The experimental lung metastasis model and the ex- perimental liver metastasis model were established by SWI990-RFP. The metastasis of pancreatic cancer ceils was assayed by whole-body fluorescence imaging system. Results We obtained stable high RFP-ex-pressing clone SWI990-RFP. The biological behavior of SW1990 and SWI990-RFP were, not cler- ent. RFP experimental metastasis models of pancreatic cancer were successfully established with the suc-cess rate being 66.7%. The metastasis and micrometastasis of orgins in early stage were. detected by using whole-body fluorescence imaging system.. Conclusion The REP gene didn' t change the biological be-havior of SWI990. The RFP metastasis models of pancreatic cancer are stable and relia ble. Key words: Pancreatic carcinoma; Green fluorescent protein; Red fluorescent protein; Me-tastasis