Establishment of a human pancreatic adenocarcinoma cell line (PSN-1) with amplifications of both c- myc and activated c-Ki- ras by a point mutation

Abstract

A human pancreatic cancer cell line, PSN-1, was established from pancreatic adenocarcinoma tissue that had been stored for 1.5 years at −80°C without any special treatment. The stored tissues were first transplanted into nude mice, and from the xenograft, the PSN-1 cell line was established. The original primary tumor and two metastatic lymph nodes were previously found to have 50-fold amplification of c- myc and also 3- to 6-fold amplification of activated c-Ki- ras with a point mutation from GGT to CGT at codon 12. PSN-1 cellsare unique in that amplifications of both c- myc and activated c-Ki- ras are present in the same degree as the original tumors. These cells were also found to contain increased amounts of c- myc and c-Ki- ras transcripts.