Establishment and application to environment sample test of novel coronavirus

Abstract

Background: Since the COVID-19 outbreak, the existence of SARS-CoV-2 virus in the air and objects surface has been proved, how to detect the novel coronavirus effectively and accurately has become the current focus. a detection method for new coronavirus in environmental sample should be established to provide basis for the protection and disinfection of novel coronavirus in the future. Methods: Environmental samples were collected primarily from isolation rooms for COVID-19 patients. In the environmental samples, the air samples are collected by bioaerosol samplers. For objects surface samples, a cotton swab was moistened with the collection liquid, and then used to wipe the surface of the object once. Results: In total, we collected 46 air samples, one of these samples' testing results was positive, which from the corridor closed to isolation rooms, and 107 object surface samples, four of these samples' testing results were positive and three were weak positive which from bathroom in the isolation rooms. Conclusions: The detection method of the novel coronavirus in environmental samples was established successfully, which provides a basis for environmental disinfection and control of the spread of new coronavirus pneumonia. And this indicated that medical staff should strengthen personal protection, strictly implement infection prevention control measures. © 2021 AME Publishing Company. All rights reserved.