Establishment and application of a multiple drug resistance gene detection method of Yersina pestis

Abstract

Objective To establishment a method for detection of multiple drug resistance gene of Yersina pestis using polymerase chain reaction (PCR), to provide a guidance for treatment of plague. Methods According to National Center for Biotechnology Information (NCBI) released sequences of aminoglycoside resistant genes of streptomycin resistant, strB, strA, beta lactam antibiotics resistant genes tem, shv, and ctx-m, sulfamilamide resistant genes sul1, sul2, and sul3, a pair of primers of each gene was designed. DNAs of 282 strains isolated from plague natural foci in Qinghai Province were amplified by PCR using every pair of primers. The products were separated using gel electrophoresis, and the results were visualized through a gel imaging system. The susceptibility of 282 Yersina pestis to streptomycin, sulfamethoxazole and ceftriaxone was tested by drug sensitivity test. Results The PCR amplification results of all samples were negative, and strains with streptomycin, sulfamilamide and beta lactam antimicrobial drug resistance genes were not found. Drug sensitivity test showed that 282 strains were highly sensitive to streptomycin, sulfamethoxazole and ceftriaxone sodium. The diameter of bacteriostasis ring > 19, 17, 21 mm, respectively. Conclusions It is a feasible method to use PCR technology to detect the multiple drug resistance genes of Yersinia pestis. Using this method to systematically monitor the resistance gene of Yersinia pestis is an efficient, economical and practical experimental method, which can provide guidance for the treatment of plague disease. Key words: Yersina pestis; Drug resistance gene; Detection method