Abstract
Background: Libanotis transcaucasica Schischk. belonging to the family of Apiaceae is a perennial plant that is distributed in Europe, northwest of Iran and also Caucasia. Purpose: Due to the interest on development of drugs from natural origins, we studied the components and cytotoxic activity of essential oil from L. transcaucasica. Methods: The essential oil of L. transcaucasica was obtained by hydro distillation of its air-dried aerial parts and was analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Cytotoxic activity of the essential oil was assessed on four human cancer cell lines (HeLa, LS180, MCF-7 and Raji). Results: The yield of oil was 0.9% (w/w) and it had yellow color. Fifty-four compounds in L. transcaucasica representing 84.0% of the oil components were identified. Germacrene B (20.2%) was the most abundant compound in this oil, followed by isospathulenol (11.0%), germacrene D (9.2%) and kessane (5.5%). The volatile oil displayed weak to moderate cytotoxic activity in all evaluated human cancer cell lines (IC50 =0.9-0.2 mg/ml). The highest and the lowest cytotoxic effects were observed on Raji and LS180 cell lines, respectively. Conclusion: Sesquiterpene hydrocarbons were identified as the main components of the essential oil (48.3%). The cytotoxic activity observed in the essential oil may be contributed to the existence of this group of hydrocarbons in the plant.
Highlights
The genus Libanotis L. belongs to the family Apiaceae
gas chromatography-mass spectrometry (GC-MS) analysis of the essential oil was performed using an Agilent 7890A chromatograph operating at 70 eV ionization energy, 0.5 s/scan and the mass range of 35-400, equipped with a HP-5 capillary column programmed as above with helium as the carrier gas with the flow rate of 0.9 ml/min and a split ratio of 1:20
The chemical composition of the essential oil of L. transcaucasica analyzed by Gas chromatography (GC) and GC-MS techniques was reported
Summary
The essential oil of L. transcaucasica was obtained by hydro distillation of its air-dried aerial parts and was analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Cytotoxic activity of the essential oil was assessed on four human cancer cell lines (HeLa, LS180, MCF-7 and Raji)
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