Abstract

Gnotobiotic (GN) and conventional (CV) colostrum-deprived lambs were fed diets deficient (D) or supplemented with. 32% of the total calories as linoleic acid (L). Plasma and organ fatty acid compositions were assessed after 2 mo. of the dietary and environmental regimens. Linoleic acid deficiency in GN and CV lambs generally resulted in decreases of linoleic acid in plasma, liver and kidney samples and increases in oleic and eicosatrienoic acids. The triene/tetraene ratios (20:3n9/20:4n6) for plasma, kidney and liver increased in the D vs L groups. However, the elevated triene/tetraene ratios observed for the L groups (<.4) indicated a slight overall essential fatty acid (EFA) deficiency condition. The presence of the microflora apparently increased the linoelic acid requirement of CV lambs. The data suggest that the requirements of the neonatal colostrum-deprived ruminant are in excess of .32% of the total calories and that changes in EFA status of the ruminant are reflected in tissue fatty acid composition.

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