Abstract
Fruit ripening is a complex phenomenon involving a series of biochemical, physiological and organoleptic changes. Ripening process in mangosteen (Garcinia mangostana Linn.) is unique of which the fruit will only ripen properly if harvested during its middle stage (emergence of purple/pink colour) but not earlier (green stage). The knowledge on the molecular mechanism and regulation behind this phenomenon is still limited. Hence, electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS) based metabolomics analysis was applied to determine the metabolome of mangosteen ripening. Specifically, mangosteen pericarp, aril and seed were collected at four different ripening stages (stage 0: green, stage 2: yellowish with pink patches, stage 4: brownish red and stage 6: dark purple) and subjected to metabolite profiling analysis. The data provided in this article have been deposited to the EMBL-EBI MetaboLights database (DOI: 10.1093/nar/gks1004. PubMed PMID: 23109552) with the identifier MTBLS595. The complete dataset can be accessed here https://www.ebi.ac.uk/metabolights/MTBLS595.
Highlights
ESI-LC-MS based-metabolomics data of mangosteen (Garcinia mangostana Linn.) fruit pericarp, aril and seed at different ripening stages
Analysed ESI-LC-MS data Mass spectrometry data was acquired from MicrOTOF-Q III (Bruker Daltonic) using an ESI negative ionisation modes Analysed data in.xlsx format Metabolites were extracted from the whole fruit of stage 0 and from the pericarp, aril and seed tissues of the three other ripening stages Plant materials were extracted using methanol acidified with formic acid solvent and 100 ppm (7 )-naringenin (m/z: 272.06) was used as an internal standard
Mangosteen fruits were harvested during its seasonal period from April until August 2016 according to the Malaysian Maturity Indices [2]
Summary
Analysed ESI-LC-MS data Mass spectrometry data was acquired from MicrOTOF-Q III (Bruker Daltonic) using an ESI negative ionisation modes Analysed data in.xlsx format Metabolites were extracted from the whole fruit of stage 0 and from the pericarp, aril and seed tissues of the three other ripening stages (stages 2, 4 and 6) Plant materials were extracted using methanol acidified with formic acid solvent and 100 ppm (7 )-naringenin (m/z: 272.06) was used as an internal standard. The data consists of molecular ions (m/z) value and its corresponding intensities, in which were detected in mangosteen pericarp, aril and seed at four different ripening stages (stages 0, 2, 4 and 6). Data was reported in.xlsx format comprising of the molecular ions (m/z) value with its corresponding retention time (RT) and intensities. Obtained datasets were normalized against internal standard (( 7)-naringenin, 100 ppm)), and subjected to log transformation (log 10) using MetaboAnalyst 3.0
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