Abstract
We have developed a novel approach to characterize protein digests by pneumatic-splitter electrospray ionization liquid chromatography mass spectrometry (PSESI-LCMS). This technique involves an interfacing of a pneumatic splitter that can dynamically generate and control a steady split flow rate of nanoliters per minute. An average PSESI-LCMS analysis, depending on the desired split ratio, consumes less than 200 femtomoles of sample. A tryptic digest map of a glycoprotein, fetuin, and a Lys-C digest map of a recombinant neurotrophin protein, neurotrophin-3, are reported here for this investigation.
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