Abstract
Nalidixic acid, the urinary antiseptic, stimulates by 40% the rate of lactate production in suspensions of human erythrocytes in Krebs bicarbonate solution at pH 7.34 and 37°C. The stimulation is evident with the other glycolytic substrates, inosine and dihydroxyacetone. Stimulation was also seen with the much poorer substrate ribose. The concentrations of ATP and 2,3-DPG in cells incubated with nalidixic acid and ouabain were consistent with changes in ATPase activity that would result in enhanced and inhibited glycolysis, respectively. Nalidixic acid, however, did not stimulate further maximally activated erythrocyte ghost ATPase whereas ouabain inhibited it. Furthermore, nalidixic acid did not overcome the ouabain inhibition. Nalidixic acid failed to stimulate glycolysis in whole erythrocyte lysates and only marginally stimulated glycolysis in erythrocytes in a medium where choline chloride replaced NaCl and KHCO 3 replaced NaHCO 3. The rate enhancement of nalidixic acid on glycolysis was interpreted as being due to an increased sodium permeability of the erythrocyte membrane in a manner similar to the effect of dinitriphenol on mitochondrial membranes.
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