Abstract
This study aimed to construct a new type of fused erythrocyte vector for gene delivery system. The conditioned medium of AD293 cells expressing vesicular stomatitis virus glycoprotein gene was collected, and erythrocyte ghost was prepared by hypotonic lysis. Using cationic polymer to condense deoxyribonucleic acid to form a complex, fusogenic erythrocyte ghost was incubated with this complex to obtain virion. Flow cytometry and luciferase activity analysis were used to detect the delivery of fusogenic erythrocyte ghost to deoxyribonucleic acid in AD293 cells and refractory cells, respectively. Transfection efficiency of fusogenic erythrocyte ghost in vivo was detected by confocal microscope. Vesicular stomatitis virus glycoprotein and erythrocyte ghost were effectively integrated, and fusogenic erythrocyte ghost was successfully prepared. deoxyribonucleic acid/polyethylenimine complexes form 100–300 nm particles. Fusogenic erythrocyte ghost can effectively incorporation deoxyribonucleic acid complexes. Confocal microscope observed red fluorescence close to blue fluorescence, indicating that labeled fusogenic erythrocyte ghost may trigger liver and spleen tissue endocytosis or fusion. A new delivery vector of fusogenic erythrocyte ghost was constructed. This system could enhance the delivery efficiency even in cells which refractory to conventional transfections in vitro.
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