Abstract
A specific targeting, biocompatible, magnetic nanoprobe for bioimaging of pancreatic cancer cell lines was developed by simple three-step route in aqueous solution. Herein, the nanocomposite consists of superparamagnetic iron oxide core having gold nanocluster adsorbed around it through electrostatic attraction. The Au NC corona layer is further conjugated with positively charged erlotinib. The Fe3O4 nanoparticles ensures the magnetic enrichment, Au NCs exhibit intense red fluorescence property and erlotinib act as a specific targeting agent for pancreatic cancer cells. The resultant targeted nanoparticles had a diameter of 24±2.5nm with relatively high fluorescence life (τav=1.17μs @ λem=640nm) and can kill EGFR over expressed pancreatic cancer cells. Confocal laser scanning microscope images of the normal cells (L-929) and pancreatic cancer cells (PANC-1) treated with probe is conducted. The results shows that the morphology of the normal cells remains intact attesting its non toxicity while PANC-1 which is over expressed with EGFR exhibit cytotoxicity towards the probe proving the potential theranostic ability of the nanocomposite.
Published Version
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