Abstract
Eosinophil production from normal human bone-marrow cells was induced by plasma or serum of patients with eosinophilia. After 10-12 d of liquid suspension culture, 14-42% of bone-marrow cells were identified as maturing eosinophil granulocytes. Upon molecular sieve chromatography, the activity eluted in a single peak corresponding to a substance of 80-85 kDa molecular weight. The activity did not stimulate colony formation in semi-solid agar media, but was detected in liquid suspension cultures only. Furthermore, addition of exogenous colony-stimulating factor (CSF) had a differential effect depending on the culture system used. In semi-solid media, predominance of eosinophil colonies was only observed when CSF was added at a low concentration. In liquid suspension cultures, however, 30-40% of cultured cells were eosinophils independent of the CSF concentration used. These findings extend recent studies in an animal model and support the hypothesis that a differentiation factor is involved in the regulation of eosinophil production in man.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
