Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures

Abstract

Eosin Nigrosin staining for assessment of sperm vitality is an essential component of basic semen analysis as it helps differentiate between dead and immotile sperms, and has clinical implications in terms of patient treatment and follow up. This staining technique involves minimal use of reagents and simple procedural steps. Standardization of the same is pertinent to warrant accurate and reproducible results in medical laboratories, even those not specialized in infertility care. We wish to share our hands on experience through various stages in implementing this staining technique from challenges faced to putting quality control processes in place as reference for peers.