Abstract
Pectic substances were extracted from inner bark of Ulmus glabra Huds. using water at 100°C. The water extract was fractionated into three polymer fractions using a combination of two different ion exchange chromatography columns (DEAE-Sepharose fast flow and PBE™ 94 (polybuffer exchanger)). The isolated pectic polymer fractions were degraded with pectolyase (endopolygalacturonase and endopectin lyase) after first having been deesterified by pectinesterase. Degradation products were fractionated by gel permeation chromatography (Bio-Gel P-2). The carbohydrate composition of the degraded products were determined by gas chromatography analysis of the trimethylsilyl methylglycoside derivatives and the glycosyl-linkages were established by methylation analysis before and after carboxyl reduction. The degradation products were also subjected to arabinogalactan-Yariv reagent crossed electrophoresis. The results show that the three pectic polymer fractions have similarities in the carbohydrate content and in the type of glycosyl-linkages present; however they react differently towards enzyme degradation and the Yariv reagent.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
