Abstract
The key enzymes of the Embden-Meyerhof-Parnas (EMP), Entner-Doudoroff (ED) and pentose phosphate (PP) pathways as well as those of the tricarboxylic acid (TCA) cycle and gluconeogenesis were assayed in the cells of Rhizobium and Bradyrhizobium grown on acetate. Except for glyceraldehyde-3-phosphate dehydrogenase, the activities of all the enzymes of the EMP, ED and PP pathways were found to be low in acetate grown cells in the present study as compared to those of glucose grown cells reported by us earlier (Mandal and Chakrabartty 1993) indicating a probable repression of the enzymes. The enzymes of the tricarboxylic acid cycle were detected in high levels in the acetate grown cells of the root-nodule bacteria. The operation of the glyoxylate pathway for acetate metabolism in the root-nodule bacteria were reported previously by us (Mandal and Chakrabartty 1992). The evidences taken together point to the simultaneous operation of both the TCA cycle and the glyoxylate cycle for acetate metabolism in these bacteria.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
