Abstract
The function of a newly sequenced protein is often estimated by sequence alignment with the sequences of proteins with known functions. However, members of a protein superfamily can share significant amino acid sequence identity but vary in the reaction catalyzed and/or the substrate used. In addition, a protein superfamily can include moonlighting proteins, which have two or more functions, and pseudoenzymes, which have a three-dimensional fold that resembles a conventional catalytically active enzyme, but do not have catalytic activity.
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