Abstract
Naturally infected sugarbeet residues mixed with soil during tillage after harvest can serve as inoculum for the leaf spot fungal pathogen Cercospora beticola when a new sugarbeet crop is planted. An enzyme-linked immunosorbent assay (ELISA) was developed in an attempt to quantify C. beticola mycelial biomass in soil. Amounts as small as 0.38 µg of freeze-dried C. beticola mycelia dispersed in carbonate buffer were detected. Fungi from different classes (Ascomycetes, Basidiomycetes, and Hyphomycetes) showed negligible cross reactivity with the polyclonal antibodies, except for the isolates of Fusarium and Trichoderma. A pre-adsorption of the antibodies was used to decrease cross reactivity of the antibodies to these isolates. Evaluation of field soil naturally infested with C. beticola showed that the assay using pre-adsorbed serum augments current detection methods and is a potential diagnostic tool for quantifying the amount of pathogen antigens in soil, therefore the potential for incidence of leaf spot disease.
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