Abstract
Protein-bound nitrotyrosine is a standard marker of nitrosative stress in vivo, its levels being associated with severity of nitrosative stress in neurological diseases. Using two commercially available antibodies we have developed protocols for an indirect competitive enzyme-linked immunosorbent assay (ELISA) aimed at detecting protein-bound nitrotyrosine. The working concentration range of the assay is 0.094–4 μg/mL, the lowest limit of detection 0.063 μg/mL, IC50 0.538 μg/mL. In a pilot study protein-bound nitrotyrosine levels in cerebrospinal fluid of acute stroke patients and in brain tissue of rats after focal ischemia were evaluated.
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