Abstract
Polymer-based monolithic materials with interconnected porous structure have received much attention as high-performance matrices for enzyme immobilization. The present work describes immobilization of horseradish peroxidase (HRP) onto a polymethacrylate-based monolith which was fabricated by thermally induced phase separation (TIPS) method. The poly(glycidyl methacrylate-co-methyl methacrylate) (PGM) monolith was modified with adipic acid dihydrazide (AADH) and ethylenediaminetetraacetic dianhydride (EDTAD) to yield carboxyl group-bearing PGM (PGM-COOH) monolith. This monolith was reacted with N-hydroxysuccinimide (NHS) to activate the carboxyl groups on the monolith and further modified with HRP. The immobilized HRP showed much higher thermal stability than the free one. Furthermore, the immobilized HRP could be reused for at least 6 times with the remaining activity of 52%. The PGM monolith may have a high potential as a solid support for enzyme immobilization.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
