Abstract
Because of the occurrence of preferential loss of human chromosomes and the existance of built-in enzyme markers, the man-mouse and man-Chinese hamster somatic cell hybrid systems have become important tools for genetic analysis of man. Cellulose acetate gel (Celloged) was found to have certain additional advantages over the other supporting media for zymogram analysis. Therefore, techniques, developed during the course of a series of investigations on somatic cell hybrids in our laboratory, are described for the electrophoretic characterization of glycose-6-phosphate dehydrogenase, nicotinamide adenine dinucleotide phosphate-dependant isocitrate dehydrogenase, lactate dehydrogenase, nicotinamide-adenine dinucleotide-dependant malate dehydrogenase, 6-phosphoglyconate dehydrogenase, indophenode oxidase (1), hypoxanthine-guanine phosphoribosyl transferase, phosphoglucomutase, and phosphoglycerate kinase 2 in the man-mouse and man-Chinese hamster somatic cell hybrid systems, on Cellogel. The results are briefly discussed.
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