Abstract
Abstract The pyrophosphatase (PPi) enzyme catalyzes the hydrolysis of pyrophosphate to orthophosphate. The PPi activity was determined in 14 hill‐land soils of the Appalachian Region. The top two horizons from each soil were sampled and stored in a field‐moist state at 4°C (4°C) and in an air‐dry (AD) state prior to determination of PPi activity. Each soil type had its own level of PPi activity. The mean PPi activities of surface and subsurface horizons stored at 4°C were 1.3 and 1.7 times higher than those of AD samples. The average PPi activities of surface horizons were 6.2 and 4.6 times higher than subsurface horizons stored at AD and 4°C. respectively. The PPi activities were positively correlated with organic C, N, forms of S and P. exchangeable cations, original moisture by weight and volume, and percent air‐filled porosity. In both surface and subsurface horizons, significant positive correlationships were observed between PPi activities and Mn content, indicating the need for Mn in the activation...
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