Abstract
In the present work, innovative composite biomaterials possessing bactericidal properties and based on the hexahistidine-tagged organophosphorus hydrolase (His6-OPH) entrapped in the poly(vinyl alcohol) cryogel (PVA-CG)/bacterial cellulose (BC) were developed. His6-OPH possesses lactonase activity, with a number of N-acyl homoserine lactones being the inducers of Gram-negative bacterial resistance. The enzyme can also be combined with various antimicrobial agents (antibiotics and antimicrobial peptides) to improve the efficiency of their action. In this study, such an effect was shown for composite biomaterials when His6-OPH was entrapped in PVA-CG/BC together with β-lactam antibiotic meropenem or antimicrobial peptides temporin A and indolicidin. The residual catalytic activity of immobilized His6-OPH was 60% or more in all the composite samples. In addition, the presence of BC filler in the PVA-CG composite resulted in a considerable increase in the mechanical strength and heat endurance of the polymeric carrier compared to the BC-free cryogel matrix. Such enzyme-containing composites could be interesting in the biomedical field to help overcome the problem of antibiotic resistance of pathogenic microorganisms.
Highlights
It is well recognized that the problem of antibiotic resistance of bacteria is of great significance [1]
bacterial cellulose (BC) samples (Figure 1a) possessing different characteristics were prepared by cultivation of Komagataeibacter xylinum B-12429 cells with fructose, glycerol, Jerusalem artichoke hydrolyzate, or beet molasses as the main carbon source (Table 1)
These results the data of other researchers [31], who pointed out that BC fibers produced with the hydrolyzate are consistent with the data of other researchers [31], who pointed out that BC fibers produced with of soybean whey had
Summary
It is well recognized that the problem of antibiotic resistance of bacteria is of great significance [1] Such resistance can progress by the mechanism of “quorum sensing” (QS), which is the ability of bacterial cells to interact with each other within the same population and to initiate a shift in the biochemical status of cells that leads to resistance. Both Gram-positive and Gram-negative (G(-)) bacteria use different signaling molecules as QS inducers. His6 -OPH in noncovalent complexes with various antimicrobial agents (antibiotic and, antimicrobial peptides) has been shown to improve the activity of each other [7,8,9]
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