Enzymatic Transesterification of DPO to Produce Biodiesel by Using Lipozyme RM IM in Ionic Liquid System

Abstract

Enzymatic transesterification reaction of degummed palm oil (DPO) in ionic liquid system has been demonstrated in producing biodiesel. Ionic liquid as a supporter of enzyme create a catalytic system that can be used in the transesterification of oil into biodiesel. Recycled Lypozime RM IM to produced biodiesel from DPO as feedstock in ionic liquid system has been investigated. In this research, synthesis of biodiesel from DPO through transesterification (using of methanol as acyl acceptor) and recycled Lipozyme RM IM as catalysts in ionic liquid [BMIM] [PF6] system is conducted in a batch reactor to obtain biodiesel as product were analyzed using Gas Chromatography (GC). As a study in this research, discussed the influence of ionic liquids to performance of Lypozime RM IM or decreasing of lipase activity while recycled that seen from the acquisition yield of biodiesel as a result. The best result in ionic liquid system with ratio of DPO to ionic liquid 1: 1.5 (molar ratio) showed that decreasing lipase activity obtained in this research was about 4.28% (yield from 68.98% to 56.12%) after 4 times with the conditions of 1:3 molar ratio, temperature of reaction 45°C and amount of Lipozyme RM IM was 30%, and 6hours reaction time.