Enzymatic synthesis of biologically active fructose-based saccharides by Aspergillus niger MK788296 levansucrase

Abstract

Fructose-based saccharides (FBS) and levans received much attention in recent years because of their diverse biological activities and safety which help in their usability in several medical and pharmaceutical applications. In the present study, FBS and levan were synthesized by Aspergillus niger MK788296 levansucrase. Fifteen fructose-based saccharides were synthesized through the interaction between levansucrase concentration (units), duration of synthesis and substrate (sucrose) concentration through a central composite design (CCD). The synthesized FBS and levans exhibited several biological activities including prebiotic, anti-microbial, fibrinolytic, antioxidant and antitumor activities. The prebiotic effect was emphasized by its promotion effect on the growth of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus plantarum DMS 20174. They also exerted antimicrobial activity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538. In addition, they exerted prominent antitumor activity against colorectal, liver and breast cancer cell lines.