Abstract
Six bacterial strains isolated from sewage waters, acclimatized on spentwash and purified were very effective in the treatment of distillery waste. Because of their apparently extensive metabolic capabilities, eight enzymes were assayed in cell free sonicates of these strains at 8, 20, 48 and 72 h during distillery waste treatment. Hexokinase (EC 2.7.1.1) was found to be a membrane bound enzyme in all the strains. Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), glucose dehydrogenase (EC 1.1.1.47) and gluconate dehydrogenase (EC 1.1.99.3) were found to be more active in the later phases of growth. Glutamate oxaloacetate aminotransferase ( l-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1), glutamate pyruvate aminotransferase ( l-alanine:2-oxoglutarate aminotransferase, EC 2.6.1.2), glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and protease were active throughout the growth period.
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