Abstract
Hydrolysis of wax esters on thin-layer chromatographic plates by porcine pancreatic lipase is described. The alcohols and free fatty acids produced were separated on the same plate, recovered and then analysed by gas—liquid chromatography for their compositions. Synthetic wax containing saturated fatty acids of various chain lengths and unsaturated fatty acids with varying numbers of methylene-interrupted cis double bonds, along with beeswax and Avicennia officinalis leaf wax were lipolysed. The results of on-plate lipolysis for 96 min compared well with those obtained by chemical hydrolysis in tubes.
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call