Abstract
The bio-treatment of double refractory gold ores (DRGO) to reduce preg-robbing needs to account for the heterogeneity of the ore so as to acquire a much more complete picture of the system. To this end, the effects of ferrous ion additives on the degradation of powdered activated carbon (PAC) by cell-free spent medium (CFSM) was studied. Au(CN)2- adsorption and Raman spectrometric results suggest that the ferrous salt could have possibly reacted with some biogenic hydrogen peroxide to aid in the degradation of PAC. The bio-treatment produced mixed solid residues containing some partially degraded aromatic compounds which were soluble in alkaline solutions. Ultimately, biodegradation of PAC using CFSM in the presence of 50 µM FeSO4.7H2O for 7 days followed by washing with 3 mM NaOH reduced Au(CN)2- uptake by 80%.
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