Enzymatic Digestion and Single Cell Isolation of Peri-implantation Stage Human Trophoblast Cells.

Abstract

Recent developments in the in vitro culture of peri-implantation stage human embryos have expanded opportunities to investigate implantation stage human development and trophoblast differentiation in the absence of maternal tissues. Emerging single cell omics analyses have offered researchers new tools to explore unanswered biological questions to new depths. In order to investigate the dynamics of human trophoblast cell differentiation during implantation at the single-cell resolution, efficient cell dissociation approaches of trophoblasts from embryos are necessary. Here, we describe the protocol for extended culture of peri-implantation stage human embryos with enzymatic digestion and manual collection of individual cells for downstream assays.