Enzymatic deacylations of esterified saccharides. II. De-esterifications of radiolabelled O-Acylglucopyranosides by mice serum and liver esterases

Abstract

1. 1. 14C-Labelled methyl 2,6-di- O- pivaloyl-α- d-glucopyranoside (1) was used as a novel substrate for esterases from mouse serum and liver. 2. 2. Stepwise de-esterification of the diester substrate 1 was achieved, and data on time-course experiments are reported. 3. 3. Kinetic studies were undertaken to compare deacylation rates for the enzymatic de-esterification of the diester substrate 1 using both, mice sera and liver microsomal fractions. 4. 4. Serum and liver esterase activities were studied in mice treated with an immunostimulating agent, peptidoglycan monomer (PGM), and a comparison made with esterases from untreated mice.