Abstract
AbstractWater‐soluble copolymers based on poly[N‐(2‐hydroxypropyl)methacrylamide] and bearing in their side chains a chromogenic substrate for chymotrypsin were prepared by direct copolymerization or polymeranalogous reaction. The substrate was L‐phenylalanine‐4′‐nitroanilide linked by its amino group to the terminal carboxylic group of the side chain. The cleavage of the substrate thus bonded, was expressed by means of the Michaelis constant, the maximum velocity, and the percentage of substrate unit accesible to the enzyme. The effect of length and chemical structure of the side chain was investigated in the first place. It was found that the chain must be longer than 6 atoms; longer chains are generally more favourable for this cleavage. The ε‐aminocaproyl group provides a chain that is most favourable for the cleavage, whereas chains with several glycyl units, though longer, are less favourable. Polymers with higher content of the substrate units undergo cleavage more readily.
Published Version
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