Environmentally friendly LLME-LTP of the bisphenol A from bovine milk samples

Abstract

This paper developed a novel and environmental microextraction for bisphenol A from milk samples using liquid-liquid microextraction with low-temperature partitioning, which is a sample clean-up step for endocrine disruptors, being a suitable alternative. Furthermore, the microextraction and determination steps were optimized to determine and quantify the bisphenol A from milk. Thus, the determination of it was made using HPLC with fluorescence detection, obtaining the analytical with a linear range from 5.0 to 150 µg L−1 bisphenol (Area = 11,894 C (µg L−1) + 22,595) with good linearity (r = 0.9983), quantification (5.0 µg L−1) limit, and variation coefficient (4.4%), which were according to the established by regulatory agencies. The bisphenol A microextraction employed only 500 µL of the bovine milk sample and 1000 µL of acetonitrile, obtaining excellent recovery values for (89 − 105%) in UHT and pasteurized milk. This proposed methodology had several advantages in determining the bisphenol A, such as quick analysis, miniaturization of the process, decreasing the solvent and sample volume, and following the Green Analytical Chemistry.