Environmental and physiological controls on the carbon isotope composition of CO2 respired by leaves and roots of a C3 woody legume (Prosopis velutina) and a C4 perennial grass (Sporobolus wrightii)

Abstract

Accurate estimates of the δ(13) C value of CO(2) respired from roots (δ(13) C(R_root) ) and leaves (δ(13) C(R_leaf) ) are important for tracing and understanding changes in C fluxes at the ecosystem scale. Yet the mechanisms underlying temporal variation in these isotopic signals are not fully resolved. We measured δ(13) C(R_leaf) , δ(13) C(R_root) , and the δ(13) C values and concentrations of glucose and sucrose in leaves and roots in the C(4) grass Sporobolus wrightii and the C(3) tree Prosopis velutina in a savanna ecosystem in southeastern Arizona, USA. Night-time variation in δ(13) C(R_leaf) of up to 4.6 ± 0.6‰ in S. wrightii and 3.0 ± 0.6‰ in P. velutina were correlated with shifts in leaf sucrose concentration, but not with changes in δ(13) C values of these respiratory substrates. Strong positive correlations between δ(13) C(R_root) and root glucose δ(13) C values in P. velutina suggest large diel changes in δ(13) C(R_root) (were up to 3.9‰) influenced by short-term changes in δ(13) C of leaf-derived phloem C. No diel variation in δ(13) C(R_root) was observed in S. wrightii. Our findings show that short-term changes in δ(13) C(R_leaf) and δ(13) C(R_root) were both related to substrate isotope composition and concentration. Changes in substrate limitation or demand for biosynthesis may largely control short-term variation in the δ(13) C of respired CO(2) in these species.