Abstract
Immature cotyledons of three inbred melon genotypes, Cucumis melo L., and all hybrid cross-combinations were placed for a 1-week treatment on liquid/membrane system prior to 5-week regeneration culture on a MS medium containing 10 μM benzyladenine. Moving plants from agar to liquid/membrane culture system altered the frequency of tetraploid regenerants. The frequency of tetraploidy was also effected by sucrose level during the 1-week liquid treatment. The most stressful conditions, those where the least regenerants were found, had the highest number frequency of tetraploidy. Genotypic variation in the modes of genetic control was observed in the different environments. Tetraploid variation can be manipulated by one week treatment of immature cotyledons prior to regeneration.
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