Abstract
We developed a new entrap-immobilization method, using a gel formationof cellulose acetate and metal (Ti, Zr) alkoxide. Several biocatalysts(β-galactosidase, α-chymotrypsine, invertase, urease, lipase andSaccharomyces cereviciae) were successfullyentrap-immobilized on this composite gel fiber. The immobilization process wassimple and the resultant immobilized biocatalysts on the gel fiber were easy tohandle. It is considered that the biocatalysts are physically entrapped amongthe gel networks and distribute throughout the gel fiber. The gel fiber wasstable in phosphate buffer solution, electrolyte solution and organic solvent,because the gel formation was due to coordination interaction between celluloseand transition metal. Therefore, it can be applicable as a support for abiotransformation in various reaction media. We examined some enzyme reactionsand biotransformation using the immobilized biocatalysts on this gel fiber andevaluated this immobilization matrix in the reactions compared to the resultsobtained by the other immobilization method. The immobilized biocatalyst showedstable activity for repeated cycles and over a long period of time. Moreover,continuous reaction could be carried out in a column reactor packed with thisimmobilized biocatalyst.
Published Version
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