Abstract
Fourteen Clostridium perfringens strains including 5 heat-resistant and 9 heat-sensitive strains were tested for enterotoxigenicity comparatively using reversed passive latex-aggultination and rabbit ileal loop tests. The results obtained by both the immunological and biological methods were in good agreement. All of the 5 heat-resistant strains were enterotoxin positive, while 3 of the 9 heat-sensitive strains were enterotoxin positive. All these heat-sensitive strains, whether enterotoxigenic or nonenterotoxigenic, produced spores in vitro as well as in vivo at approximately the same frequency as did the heat-resistant strains, indicating that absence of enterotoxin was not due to inability of these strains to produce spores.
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