Abstract
Number of enterococci determined in raw milk cistern samples was in range of 2.95 to 4.18 log CFU.ml-1 and raw milk samples obtained from storage tanks contained enterococci count in the range of 3.04 to 4.51 log CFU.ml-1. Results of microbiological quality evaluation showed, that count of enterococci increased during cold storage of raw milk. Portion of enterococci from the total microflora of raw milk taken from cistern samples was 0.44 %, otherwise in raw milk samples taken from storage tanks portion of enterococci decreased to 0.38 %. Among enterococci isolates E. faecalis was the predominat species in tested samples of raw milk from both cistern – 58.1 % and storage tank – 71.7 %. The following species were identified E. faecium, E. group III., E. mundtii, E. casseliflavus. It was found that 38 % E. faecalis isolates were able to form a biofilm.
Highlights
Number of enterococci determined in raw milk cistern samples was in range
raw milk samples obtained from storage tanks contained enterococci count in the range
that count of enterococci increased during cold storage of raw milk
Summary
Enterokoky sa stanovili pri teplote 37 ± 1 °C na živnom médiu Slanetz-Bartley (HiMedia, India) po dobu kultivácie 48 ± 2 hod (STN 56 0100, 1970). Suspektné kolónie enterokokov izolované zo vzoriek mlieka sa preočkovali čiarovaním na selektívne médium so žlčou a eskulínom (BEA agar) (HiMedia, India) a inkubovali sa pri teplote 37 ± 1 °C po dobu 24 ± 2 hod. Enterokoky sa naočkovali do tryptón-sójového bujónu (TSB), živného bujónu (ŽB) a MRS bujónu (keďže jedným zo základných faktorov pre tvorbu biofilmu je substrát) a inkubovali sa pri teplote 37 ± 1 °C po dobu 20 – 24 hod. Na kvantifikáciu tvorby biofilmu sa použila metóda farbenia s kryštálovou violeťou modifikovaná podľa Koreňovej et al (2008). Sledované druhy enterokokov sú schopné tvorby biofilmu, ak optická denzita zostávajúcich buniek dosiahne po odčítaní hodnoty
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