Enriched autoinducer-2 (AI-2)-based quorum quenching consortium in a ceramic anaerobic membrane bioreactor (AnMBR) for biofouling retardation

Abstract

This study is the first to enrich a facultative QQ consortium for AI-2-based quorum sensing (QS) disruption (FQQ2) and discover its quorum quenching (QQ) performance in an anaerobic membrane bioreactor (AnMBR) for membrane fouling retardation. Herein, FQQ2 was enriched by the enrichment culture using 4,5-dihydroxy-2,3-pentanedione (DPD) followed by anaerobic screening. FQQ2 was composed of various facultative AI-2-based QQ microorganisms including Acinetobacter, Comamonas, Stenotrophomonas, and FQQ2 was capable to degrade 96.96% of DPD in 9 h. More importantly, FQQ2 prolonged membrane filtration operation by an average of 3.72 times via reduction of DPD in the AnMBR treating domestic wastewater (p ≤ 0.05). QQ was implicated to reduce the content of proteins and carbohydrates of the extracellular polymeric substances (EPS) of suspended biomass by 24.16% and 10.39%, respectively, and concentration of proteins of the soluble microbial products (SMP) by 18.77%. Parallel factor (PARAFAC) modelling of excitation-emission matrix (EEM) demonstrated that QQ could reduce the content of fulvic acid-like and humic acid-like substances, aromatic proteins and soluble-microbial-by-product-like proteins of the EPS (p ≤ 0.05) and abate the content of soluble-microbial-by-product-like proteins in the SMP (p ≤ 0.05). The lower EPS content of suspended biomass could be rendered with the reduced relative abundance of AI-2-regulated Christensenellaceae;g-, Hyphomicrobium, Leucobacter and Microbacterium by 48.48%, 76.56%, 64.78% and 59.26%, respectively, and QQ led to the reduction of the relative abundance of Christensenellaceae;g- and Leucobacter in the cake layer by 31.07% and 51.43%, respectively. Moreover, quantity of organics as well as planktonic microorganisms in the supernatant decreased in presence of FQQ2 (p ≤ 0.05). Of note, markedly lower relative abundance of AI-2-regulated Sulfurovum in supernatant by 97.74% resulted in its lower abundance of cake layer. Intriguingly, in the presence of QQ, methane production was statistically enhanced by 62.5% (p ≤ 0.05). It was closely linked to the decrease of sulfate reduction (p ≤ 0.05), which resulted from 37.93% lower abundance of sulfate-reduction Desulfomonile in the suspended biomass (p ≤ 0.05). Collectively, this study sheds lights on the development of AI-2-based QQ for biofouling control in AnMBRs.