Feasibility of isolated novel facultative quorum quenching consortiums for fouling control in an AnMBR

Abstract

Anaerobic membrane bioreactor (AnMBR) technology is being recognized as an appealing strategy for wastewater treatment, however, severity of membrane fouling inhibits its widespread implementations. This study engineered novel facultative quorum quenching consortiums (FQQs) coping with membrane fouling in AnMBRs with preliminary analysis for their quorum quenching (QQ) performances. Herein, Acyl-homoserine lactones (AHLs)-based quorum sensing (QS) in a lab-scale AnMBR initially revealed that N-Hexanoyl-dl-homoserine lactone (C6-HSL), N-Octanoyl-dl-homoserine lactone (C8-HSL) and N-Decanoyl-dl-homoserine lactone (C10-HSL) were the dominant AHLs in AnMBRs in this study. Three FQQs, namely, FQQ-C6, FQQ-C8 and FQQ-C10, were harvested after anaerobic screening of aerobic QQ consortiums (AeQQs) which were isolated by enrichment culture, aiming to degrade C6-HSL, C8-HSL and C10-HSL, respectively. Growth of FQQ-C6 and FQQ-C10 using AHLs as carbon source under anaerobic condition was significantly faster than those using acetate, congruously suggesting that their QQ performance will not be compromised in AnMBRs. All FQQs degraded a wide range of AHLs pinpointing their extensive QQ ability. FQQ-C6, FQQ-C8 and FQQ-C10 remarkably alleviated extracellular polymeric substances (EPS) production in a lab-scale AnMBR by 72.46%, 35.89% and 65.88%, respectively, and FQQ-C6 retarded membrane fouling of the AnMBR by 2 times. Bioinformatics analysis indicated that there was a major shift in dominant species from AeQQs to FQQs where Comamonas sp., Klebsiella sp., Stenotrophomonas sp. and Ochrobactrum sp. survived after anaerobic screening and were the majority in FQQs. High growth rate utilizing AHLs under anaerobic condition and enormous EPS retardation efficiency in FQQ-C6 and FQQ-C10 could be attributed to Comamonas sp.. These findings demonstrated that FQQs could be leveraged for QQ under anaerobic systems. We believe that this was the first work proposing a bacterial pool of facultative QQ candidates holding biotechnological promises for membrane fouling control in AnMBRs.