Abstract
A new spectrofluorimetric method was developed for the determination of trace amounts of coenzyme A using enoxacin–Tb3+ as a fluorescent probe. In the presence of periodic acid (H5IO6), coenzyme A could remarkably enhance the fluorescence intensity of the Tb3+–enoxacin complex at 545 nm at pH 5.4. The optimal conditions for the determination of coenzyme A were also investigated. This method could be successfully applied to assess coenzyme A in injection and biological samples. Moreover, the enhancement mechanism of the fluorescence intensity of the coenzyme A–Tb3+–enoxacin system in the presence of H5IO6 was also discussed.
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